This study focused on the analysis of diastase activity, hydroxymethylfurfural, moisture content and electrical conductivity in honey samples from stores in Brno. We collected and analysed 34 honey samples. We proceeded in accordance with the “Harmonised Methods of the International Honey Commission” (Bogdanov 2009). Three of the blossom honey samples were labelled “Czech Honey”. Of the parameters analysed, only diastase activity was found to be in noncompliance with the value of minimal diastase activity set forth by Decree No. 76/2003 Coll. and Council Directive 2001/110/EC. All the analysed samples met the hydroxymethylfurfural concentration, moisture content and electrical conductivity according to legislative values, except for one “Czech Honey” sample with a moisture content and hydroxymethylfurfural concentration exceeding the limit value according to Quality Standard for Czech Honey No. 1/1999.
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